Recombinant Human 26S Proteasome, Ub-VS treated

• Cat.No.: 26SPro-131H
• Product Overview: Recombinant Human 26S Proteasome was expressed in Transformed HEK293 cells. The 26S proteasome associated DUB activity was removed through washing and treatment with ubiquitin–vinylsul-phone (Ub–VS) which forms an adduct with the active site cysteine in DUBs of the thiol protease class (Lee, et al., 2010).

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: The ubiquitin–proteasome system (UPS) targets selected proteins for degradation by the 26S proteasome. The initial steps in this pathway generate proteins that are covalently tagged with a polyubiquitin chain that is then recognized by ubiquitin receptors of the 26S proteasome.
• Form: 50mM Tris/HCl pH7.4, 10% Glycerol, 1mM ATP
• Molecular Mass: 2500kDa
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.2mg/ml