Recombinant human 5'-AMP-activated protein kinase catalytic subunit alpha-1 K45R mutant protein, His-tagged

• Cat.No.: PRKAA1 K45R-01H
• Product Overview: Recombinant human 5'-AMP-activated protein kinase catalytic subunit alpha-1 K45R mutant protein with N-terminal His6 fusion tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 566
• Description: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Form: Buffered aqueous solution
• Molecular Mass: 65 kDa
• AA Sequence: MHHHHHHMRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGRHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMCANLIKILAQ
• Purity: ＞85% by SDS-PAGE
• Applications: ELISA
• Storage: Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles
• Concentration: 0.136 mg/ml
• Storage Buffer: Solution in PBS, pH7.4, 0.1% Sodium Lauroyl Sarcosinate

Gene Information

• Gene Name: PRKAA1
• Official Symbol: PRKAA1
• Synonyms: Acetyl-CoA carboxylase kinase (ACACA kinase);
  Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase);
  Tau-protein kinase PRKAA1.
• Gene ID: 5562
• mRNA Refseq: NM_006251.6
• Protein Refseq: NP_006242.5
• MIM: 602739
• UniProt ID: Q13131