Recombinant Human AAK1 Protein, GST-tagged

• Cat.No.: AAK1-017H
• Product Overview: Human AAK1 full-length ORF ( AAH02695.1, 1 a.a. - 474 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 78.4 kDa
• AA Sequence: MKKFFDSRREQGGSGLGSGSSGGGG STSGLGSGYIGRVFGIGRQQVTVDE VLAEGGFAIVFLVRTSNGMKCALKR MFVNNEHDLQVCKREIQIMRDLSGH KNIVGYIDSSINNVSSGDVWEVLIL MDFCRGGQVVNLMNQRLQTGFTENE VLQIFCDTCEAVARLHQCKTPIIHR DLKVENILLHDRGHYVLCDFGSATN KFQNPQTEGVNAVEDEIKKYTTLSY RAPEMVNLYSGKIITTKADIWALGC LLYKLCYFTLPFGESQVAICDGNFT IPDNSRYSQDMHCLIRYMLEPDPDK RPDIYQVSYFSFKLLKKECPIPNVQ NSPIPAKLPEPVKASEAAAKKTQPK ARLTDPIPTTETSIAPRQRPKAGQT QPNPGILPIQPALTPRKRATVQPPP QAAGSSNQPGLLASVPQPKPQAPPS QPLPQTQAKQPQAPPTPQQTPSTQA QGLPAQAQATPQHQQHTIKLSMKL
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: AAK1 AP2 associated kinase 1 [ Homo sapiens ]
• Official Symbol: AAK1
• Synonyms: AAK1; AP2 associated kinase 1; AP2-associated protein kinase 1; DKFZp686K16132; KIAA1048; adaptor-associated kinase 1; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; MGC138170; MGC164568; MGC164570; DKFZp686F03202
• Gene ID: 22848
• mRNA Refseq: NM_014911
• Protein Refseq: NP_055726
• UniProt ID: Q2M2I8