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Recombinant Human ABCG4 protein, His & T7-tagged

Cat.No. : ABCG4-8153H
Product Overview : Recombinant Human ABCG4 aa. (Val59~Thr301 (Accession # Q9H172)) fused with N-terminal His & T7 tag was produced in E. coli cells.
  • Specification
  • Gene Information
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Source : E. coli
Species : Human
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 30.7kDa.
Protein length : Val59~Thr301 (Accession # Q9H172)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 9.3.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage Buffer : Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Reconstitution : Reconstitute in sterile ddH2O.
Gene Name : ABCG4 ATP binding cassette subfamily G member 4 [ Homo sapiens (human) ]
Official Symbol : ABCG4
Synonyms : ABCG4; ATP binding cassette subfamily G member 4; WHITE2; ATP-binding cassette sub-family G member 4; ATP-binding cassette, sub-family G (WHITE), member 4; putative ABC transporter
Gene ID : 64137
mRNA Refseq : NM_001142505.1
Protein Refseq : NP_001135977.1
UniProt ID : Q9H172

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (21)

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What is the relationship between intracellular glutathione (GSH) levels and the regulation of ABCG4 expression? 05/14/2022

ABCG4 expression was regulated redox-dependently by intracellular glutathione (GSH) levels

What is the role of ABCG4 in neurodegenerative diseases? 04/30/2022

ABCG4 has been implicated in neurodegenerative diseases, including Alzheimer's disease. It is thought to be involved in the efflux of lipids and cholesterol from brain cells, contributing to lipid homeostasis and potentially influencing the pathogenesis of these diseases. 

What is the role of ABCG1 and ABCG4 heterodimerization in cellular processes? 09/14/2021

The heterodimerization of ABCG1 and ABCG4 indicates their ability to interact and form complexes. This interaction might be essential for their proper function or regulation. 

What is the impact of doxorubicin-induced upregulation of ABCG4 on prostate cancer chemoresistance? 04/07/2021

Doxorubicin (Dox)‐induced ABCG4 upregulation causes prostate cancer (PCa) chemoresistance

Are there any known substrates of ABCG4 in the context of drug resistance? 01/04/2021

The exact substrates of ABCG4 in the context of drug resistance are still being investigated. While ABCG4's involvement in mediating drug resistance has been established, the specific drugs or compounds that it transports have not been conclusively identified. 

How do glutathione levels influence the expression of ABCG4 in androgen-independent prostate cancer cells? 12/02/2020

Glutathione levels dictate ABCG4 expression in androgen‐independent PCa cells.

Are there any known genetic or epigenetic factors that influence ABCG4-mediated drug resistance? 09/17/2020

The specific genetic or epigenetic factors influencing ABCG4-mediated drug resistance have not been extensively studied. However, it is possible that genetic variations or alterations in epigenetic marks could impact ABCG4 expression or function, potentially contributing to drug resistance.

Can ABCG4 expression be modulated by therapeutic interventions in NSCLC? 04/28/2020

Strategies such as targeted therapies or combination treatments may be investigated to inhibit ABCG4 expression or activity, with the aim of overcoming drug resistance and improving treatment outcomes.

 How do statins sensitize androgen-independent prostate cancer cells to doxorubicin, and what role do they play in inhibiting ABCG4 levels? 02/06/2020

Statins sensitize androgen‐independent PCa cells to Dox by inhibiting ABCG4 levels.

How does the induction of ABCG4 contribute to chemoresistance in androgen-independent prostate cancer cells treated with doxorubicin or cisplatin? 01/21/2020

In the present study, we documented that doxorubicin (Dox) or cisplatin‐induced prostate cancer (PCa) chemoresistance is predominantly mediated by the induction of ABCG4 in androgen‐independent PCa cells.

How is ABCG4 expression regulated in non-small-cell lung cancer (NSCLC)? 09/22/2019

The regulation of ABCG4 expression in NSCLC is not fully understood. However, studies suggest that various factors, including transcriptional regulators, signaling pathways, and microenvironmental cues, can influence ABCG4 expression.

 Are there any genetic variants of ABCG4 associated with disease susceptibility? 09/02/2019

The presence of genetic variants or mutations in ABCG4 has not been extensively studied for disease susceptibility. However, it is possible that variations in the ABCG4 gene sequence could affect its function or expression, potentially influencing disease susceptibility.

Does ABCG4-mediated drug resistance involve efflux of specific drugs or a broader range of compounds? 03/14/2019

ABCG4-mediated drug resistance may involve the efflux of specific drugs or a broader range of compounds. The substrate specificity of ABCG4 is still being elucidated, and it is possible that it can transport multiple drugs or compounds.

Can targeting ABCG4 be a potential strategy to overcome drug resistance in NSCL? 02/07/2019

Targeting ABCG4 is being explored as a potential strategy to overcome drug resistance in NSCLC. By inhibiting or modulating ABCG4 activity, it may be possible to prevent drug efflux and enhance the effectiveness of chemotherapy.

What are the specific mechanisms by which ABCG4 contributes to drug resistance in NSCLC? 09/14/2018

The specific mechanisms by which ABCG4 contributes to drug resistance in NSCLC are not yet fully understood. However, it is hypothesized that ABCG4 actively transports drugs out of cancer cells, preventing their accumulation and reducing their cytotoxic effects. 

How does the sequence similarity between ABCG1 and ABCG4 contribute to their function? 07/06/2018

The sequence similarity of 69% between ABCG1 and ABCG4 suggests a close evolutionary relationship and potential functional similarities. This similarity may indicate shared structural and functional features that enable them to perform similar roles in cellular processes.

How is the expression or activity of ABCG4 regulated in the context of drug resistance? 04/17/2018

The regulation of ABCG4 expression and activity in the context of drug resistance is not yet fully understood. It is likely that various factors, such as genetic and epigenetic mechanisms, signaling pathways, and microenvironmental cues, influence ABCG4 expression and activity.

Can you explain the involvement of ABCG4 in mediating drug resistance in non-small-cell lung cancer (NSCLC)? 06/21/2017

ABCG4 has been implicated in mediating drug resistance in non-small-cell lung cancer (NSCLC). It is likely that ABCG4 functions as an efflux transporter, pumping out anti-cancer drugs from cancer cells and reducing their effectiveness. This can contribute to treatment resistance and hinder the success of chemotherapy.

What is the regulatory mechanism involving NF-κB, c-Myc, and cAMP-regulatory element-binding protein that controls ABCG4 expression in androgen-independent prostate cancer cells? 08/14/2016

ABCG4 is regulated via NF‐kB‐c‐Myc‐cAMP‐regulatory element‐binding protein axis in androgen‐independent PCa cells.

How does ABCG4 play a role in doxorubicin-mediated chemoresistance in prostate cancer, and what is its potential as a therapeutic target? 02/23/2016

ABCG4 play a role in Dox-mediated chemoresistance, and as a potential therapeutic target in drug-induced PCa 

What is the impact of ABCG4 expression on cellular cholesterol homeostasis? 01/26/2016

ABCG4 plays a role in cellular cholesterol homeostasis by facilitating the efflux of cholesterol from cells. Its expression levels can affect the balance between cholesterol influx and efflux, thereby influencing cellular cholesterol levels and lipid metabolism.

Customer Reviews (5)

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Reviews
02/18/2022

    The availability of online resources, including FAQs and troubleshooting guides, proved helpful when encountering any challenges during my research.

    03/13/2021

      he protein product maintained its activity over an extended period, allowing for long-term experiments without compromising performance

      10/05/2020

        I conducted a side-by-side comparison with other protein products, and the superior quality of this product was evident in terms of purity and functionality

        09/30/2017

          The company provided technical datasheets, protocols, and application notes.

          03/25/2016

            The protein product underwent stringent quality control tests, ensuring the absence of endotoxins and other contaminants that could affect my assays

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