Recombinant Human ACBD5 293 Cell Lysate
- Gene Information
- Related Products
|Description :||Antigen standard for acyl-Coenzyme A binding domain containing 5 (ACBD5), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.|
|Source :||HEK 293 cells|
|Components :||This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).|
|Size :||0.1 mg|
|Storage Instruction :||Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.|
|Applications :||ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.|
|Gene Name :||ACBD5 acyl-CoA binding domain containing 5 [ Homo sapiens ]|
|Official Symbol :||ACBD5|
|Synonyms :||ACBD5; acyl-CoA binding domain containing 5; acyl Coenzyme A binding domain containing 5; acyl-CoA-binding domain-containing protein 5; DKFZp434A2417; KIAA1996; endozepine-related protein; acyl-Coenzyme A binding domain containing 5; membrane-associated diazepam binding inhibitor;|
|Gene ID :||91452|
|mRNA Refseq :||NM_001042473|
|Protein Refseq :||NP_001035938|
|UniProt ID :||Q5T8D3|
|Chromosome Location :||10p12.1|
|Function :||binding; fatty-acyl-CoA binding; lipid binding;|
|◆ Recombinant Protein|
|ACBD5-1033H||Recombinant Human ACBD5 Protein (1-461 aa), His-SUMO-tagged||+Inquiry|
|Acbd5-1491M||Recombinant Mouse Acbd5 Protein, Myc/DDK-tagged||+Inquiry|
|ACBD5-101R||Recombinant Rat ACBD5 Protein, His (Fc)-Avi-tagged||+Inquiry|
|ACBD5-3132B||Recombinant Bovine ACBD5, His-tagged||+Inquiry|
|ACBD5-446R||Recombinant Rat ACBD5 Protein||+Inquiry|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
We are offering a 10% discount on all products for a limited time. The clock is ticking down. Be sure to take advantage of our holiday promotions!
Yes, we offer custom protein production services, and you can describe in detail the protein length that meets your requirements when submitting the online inquiry form.
It can be used for various applications in research and drug discovery, such as studying its role in protein trafficking and sorting, lipid metabolism and signaling, and disease pathogenesis. It can also be used as an antigen in antibody production, and as a tool for screening and identifying potential drug candidates that target ACBD5-mediated pathways.
As a supplier with an excellent reputation, Creative BioMart offers product troubleshooting, assay optimization, and protocol development services, as well as online resources such as datasheets, protocols, and FAQs.
One limitation of using recombinant ACBD5 protein is the potential for protein aggregation or denaturation during storage or handling, which may affect its biological activity or structure. Additionally, the use of recombinant protein may not fully recapitulate the complexity and diversity of native ACBD5 protein interactions and functions in vivo.
We support custom protein production, and you can choose the type and position of the tag you need when submitting the online inquiry form.
Yes, we can offer customization or modification services, such as site-directed mutagenesis or fusion with other tags or proteins.
Yes, and please furnish our team of scientists with a comprehensive description of the ACBD5 protein planned for deployment in the synthesis of the primary antibody. This includes essential details pertaining to its species, amino acid residue sequence and fusion tag. Subsequently, our team of experts will carefully evaluate the experimental prerequisites and provide customized solutions accordingly.
The biological activity of recombinant ACBD5 protein may be validated using different assays depending on its specific function. For example, its role in protein trafficking and sorting may be studied using assays such as vesicle trafficking assays or immunofluorescence staining. The validation of the protein's biological activity may also involve comparing its activity to that of native ACBD5 protein or other known ACBD5-interacting proteins.
Yes, it can be used in structural biology studies, such as X-ray crystallography, to determine its three-dimensional structure. However, obtaining high-quality crystals may require optimization of the protein expression, purification, and crystallization conditions.
Customer Reviews (3)Write a review
The recombinant chicken ACBD5 protein was easy to work with and produced consistent results in our experiments. -
I had a great experience purchasing the Recombinant Mouse Acbd5 Protein from Creative BioMart. Their customer service was exceptional. -
I highly recommend Creative BioMart's Recombinant ACBD5 Protein for anyone conducting research in this field. -
Ask a Question for All ACBD5 Products
Required fields are marked with *
My Review for All ACBD5 Products
Required fields are marked with *