Recombinant Human ACO1 Protein, GST-Tagged

• Cat.No.: ACO1-163H
• Product Overview: Human ACO1 full-length ORF ( NP_002188.1, 1 a.a. - 889 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 124.8 kDa
• AA Sequence: MSNPFAHLAEPLDPVQPGKKFFNLN KLEDSRYGRLPFSIRVLLEAAIRNC DEFLVKKQDIENILHWNVTQHKNIE VPFKPARVILQDFTGVPAVVDFAAM RDAVKKLGGDPEKINPVCPADLVID HSIQVDFNRRADSLQKNQDLEFERN RERFEFLKWGSQAFHNMRIIPPGSG IIHQVNLEYLARVVFDQDGYYYPDS LVGTDSHTTMIDGLGILGWGVGGIE AEAVMLGQPISMVLPQVIGYRLMGK PHPLVTSTDIVLTITKHLRQVGVVG KFVEFFGPGVAQLSIADRATIANMC PEYGATAAFFPVDEVSITYLVQTGR DEEKLKYIKKYLQAVGMFRDFNDPS QDPDFTQVVELDLKTVVPCCSGPKR PQDKVAVSDMKKDFESCLGAKQGFK GFQVAPEHHNDHKTFIYDNTEFTLA HGSVVIAAITSCTNTSNPSVMLGAG LLAKKAVDAGLNVMPYIKTSLSPGS GVVTYYLQESGVMPYLSQLGFDVVG YGCMTCIGNSGPLPEPVVEAITQGD LVAVGVLSGNRNFEGRVHPNTRANY LASPPLVIAYAIAGTIRIDFEKEPL GVNAKGQQVFLKDIWPTRDEIQAVE RQYVIPGMFKEVYQKIETVNESWNA LATPSDKLFFWNSKSTYIKSPPFFE NLTLDLQPPKSIVDAYVLLNLGDSV TTDHISPAGNIARNSPAARYLTNRG LTPREFNSYGSRRGNDAVMARGTFA NIRLLNRFLNKQAPQTIHLPSGEIL DVFDAAERYQQAGLPLIVLAGKEYG AGSSRDWAAKGPFLLGIKAVLAESY ERIHRSNLVGMGVIPLEYLPGENAD ALGLTGQERYTIIIPENLKPQMKVQ VKLDTGKTFQAVMRFDTDVELTYFL NGGILNYMIRKMAK
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ACO1 aconitase 1, soluble [ Homo sapiens ]
• Official Symbol: ACO1
• Synonyms: ACO1; aconitase 1, soluble; IREB1; cytoplasmic aconitate hydratase; IREBP; IRP1; IRE-BP 1; aconitate hydratase; citrate hydro-lyase; iron regulatory protein 1; ferritin repressor protein; iron-responsive element binding protein 1; iron-responsive element-binding protein 1; ACONS; IREBP1
• Gene ID: 48
• mRNA Refseq: NM_002197
• Protein Refseq: NP_002188
• MIM: 100880
• UniProt ID: P21399