Recombinant Human ACO1, GST-tagged

• Cat.No.: ACO1-9294H
• Product Overview: Recombinant Human ACO1 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: C-term-350a.a.
• Description: Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5 UTR of ferritin mRNA, and in the 3 UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: ACO1 aconitase 1, soluble [ Homo sapiens ]
• Official Symbol: ACO1
• Synonyms: ACO1; aconitase 1, soluble; IREB1; cytoplasmic aconitate hydratase; IREBP; IRP1; IRE-BP 1; aconitate hydratase; citrate hydro-lyase; iron regulatory protein 1; ferritin repressor protein; iron-responsive element binding protein 1; iron-responsive element-binding protein 1; ACONS; IREBP1
• Gene ID: 48
• mRNA Refseq: NM_002197
• Protein Refseq: NP_002188
• MIM: 100880
• UniProt ID: P21399
• Chromosome Location: 9p21.1
• Pathway: Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Citrate cycle, first carbon oxidation, oxaloacetate => 2-oxoglutarate, organism-specific biosystem; Citrate cycle, first carbon oxidation, oxaloacetate => 2-oxoglutarate, conserved biosystem; Glyoxylate and dicarboxylate metabolism, organism-specific biosystem
• Function: 4 iron, 4 sulfur cluster binding; 4 iron, 4 sulfur cluster binding; RNA binding; RNA binding; aconitate hydratase activity; aconitate hydratase activity; citrate hydro-lyase (cis-aconitate-forming) activity; iron-responsive element binding; iron-responsiv