Recombinant Human ADAMTS13 protein, His & T7-tagged

• Cat.No.: ADAMTS13-5660H
• Product Overview: Recombinant Human ADAMTS13 aa. (Pro682~Cys859 (Accession # Q76LX8)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.3kDa
• Protein length: Pro682~Cys859 (Accession # Q76LX8)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 4.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: ADAMTS13 ADAM metallopeptidase with thrombospondin type 1 motif 13 [ Homo sapiens (human) ]
• Official Symbol: ADAMTS13
• Synonyms: ADAMTS13; ADAM metallopeptidase with thrombospondin type 1 motif 13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13; A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13; vWF-cleaving protease; von Willebrand factor-cleaving protease
• Gene ID: 11093
• mRNA Refseq: NM_139025.4
• Protein Refseq: NP_620594.1
• UniProt ID: Q76LX8