Recombinant Human ADP-ribosylation Factor 1, His-tagged

• Cat.No.: ARF1-2769H
• Product Overview: Recombinant human ARF1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 22.8 kDa (201aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: ARF1, also known as ADP-ribosylation factor 1, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. It is essential and ubiquitous in eukaryotes, as this protein is involved in vesicular transport and functioning via phospholipase D activation. ARF1 plays a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT.
• Purity: > 90% by SDS - PAGE.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTIL YK LKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGGQDKIRPLWRH YFQNTQGLIF VV DSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKL GLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Vibrio cholerae infection; HIV Infection; Membrane Trafficking

Gene Information

• Gene Name: ARF1 ADP-ribosylation factor 1 [ Homo sapiens ]
• Synonyms: ADP-ribosylation factor 1; ARF1
• Gene ID: 375
• mRNA Refseq: NM_001024226
• Protein Refseq: NP_001019397
• MIM: 103180
• UniProt ID: P84077
• Chromosome Location: 1q42
• Function: GTP binding; GTPase activity; nucleotide binding; protein binding; receptor signaling protein activity