Recombinant Human AGRN protein, His/T7-tagged

• Cat.No.: AGRN-12H
• Product Overview: Recombinant Human AGRN fused with His/T7 tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His/T7
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 64.2kDa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF LVEKS AGDVDTLAFD GRTFVEYLNA VTESEKALQS NHFELSLRTE ATQGLVLWSG KATERADYVA LAIVDGHLQL SYNLGSQPVV LRSTVPVNTN RWLRVVAHRE QREGSLQVGN EAPVTGSSPL GATQLDTDGA LWLGGLPELP VGPALPKAYG TGFVGCLRDV VVGRHPLHLL EDAVTKPELR PCPTP
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: AGRN agrin [ Homo sapiens ]
• Official Symbol: AGRN
• Synonyms: AGRN; agrin; AGRIN; agrin proteoglycan
• Gene ID: 375790
• mRNA Refseq: NM_198576
• Protein Refseq: NP_940978
• MIM: 103320
• UniProt ID: O00468
• Chromosome Location: 1p36.33
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem; NCAM1 interactions, organism-specific biosystem
• Function: acetylcholine receptor regulator activity; laminin binding; protein binding; structural constituent of cytoskeleton