Active Recombinant Human AIM2 protein, GST-tagged

• Cat.No.: AIM2-1159H
• Product Overview: Active Recombinant Human AIM2 protein(NP_004824.1) (Met 1-Thr 343) was expressed in Insect Cells, fused with the GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 1-343 aa
• Form: Lyophilized from sterile 50mM Tris, 1M NaCl, 0.5mM PMSF, 5mM GSH, pH 8.0.
• Bio-activity: Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells（Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 4-7 μg/ml.
• Molecular Mass: The recombinant human AIM2/GST chimera consists of 567 amino acids and predicts a molecular mass of 65.2 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: AIM2 absent in melanoma 2 [ Homo sapiens ]
• Official Symbol: AIM2
• Synonyms: AIM2; absent in melanoma 2; interferon-inducible protein AIM2; PYHIN4
• Gene ID: 9447
• mRNA Refseq: NM_004833
• Protein Refseq: NP_004824
• MIM: 604578
• UniProt ID: O14862