Recombinant Human AKT1 Protein, His-tagged, Alexa Fluor 488 conjugated

• Cat.No.: AKT1-786HAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant human AKT1 (NP_001014431.1) (Met 1-Ala 480), fused with a polyhistidine tag at the C-terminus, was produced in Baculovirus-Insect cells.

Specification

• Source: Insect cells
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: The recombinant human AKT1 consists of 491 amino acids and migrates as an approximately 57 kDa band as predicted in SDS-PAGE under reducing conditions.
• Protein length: 491
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 3 mM DTT, 0.5 mM PMSF, 5% Glycerol, pH 8.0
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: AKT1 v-akt murine thymoma viral oncogene homolog 1 [ Homo sapiens ]
• Official Symbol: AKT1
• Gene ID: 207
• mRNA Refseq: NM_001014431
• Protein Refseq: NP_001014431
• MIM: 164730
• UniProt ID: P31749