Active Recombinant Human ALCAM protein, His-Fc-tagged

• Cat.No.: ALCAM-3241H
• Product Overview: Active Recombinant Human ALCAM protein is expressed with sequence (Met1-Ala526) of human ALCAM/CD166 (Accession #NP_001618.2) fused with an Fc, 6xHis tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-526 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA.Immobilized Human CD6 at 1 μg/mL (100 μL/well) can bind Human CD166 with a linear range of 0.05-1.2 ng/mL. 2.Measured by its ability to block adhesion of Jurkat cells to immobilized Recombinant Human CD6 Protein . The ED50 for this effect is 2.7-10.8 μg/mL.
• Molecular Mass: 85.57 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C. Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: ALCAM activated leukocyte cell adhesion molecule [ Homo sapiens ]
• Official Symbol: ALCAM
• Synonyms: ALCAM; activated leukocyte cell adhesion molecule; activated leucocyte cell adhesion molecule; CD166 antigen; CD166; MEMD; FLJ38514; MGC71733
• Gene ID: 214
• mRNA Refseq: NM_001243280
• Protein Refseq: NP_001230209
• MIM: 601662
• UniProt ID: Q13740