Recombinant Human ALPL Protein, GST-tagged

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Recombinant Human ALPL Protein, GST-tagged

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Cat.No. : ALPL-494H
Product Overview : Human ALPL full-length ORF ( NP_000469.3, 1 a.a. - 524 a.a.) recombinant protein with GST-tag at N-terminal.
Description : This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 83.7 kDa
AA Sequence : MISPFLVLAIGTCLT NSLVPEKEKDPKYWR DQAQETLKYALELQK LNTNVAKNVIMFLGD GMGVSTVTAARILKG QLHHNPGEETRLEMD KFPFVALSKTYNTNA QVPDSAGTATAYLCG VKANEGTVGVSAATE RSRCNTTQGNEVTSI LRWAKDAGKSVGIVT TTRVNHATPSAAYAH SADRDWYSDNEMPPE ALSQGCKDIAYQLMH NIRDIDVIMGGGRKY MYPKNKTDVEYESDE KARGTRLDGLDLVDT WKSFKPRYKHSHFIW NRTELLTLDPHNVDY LLGLFEPGDMQYELN RNNVTDPSLSEMVVV AIQILRKNPKGFFLL VEGGRIDHGHHEGKA KQALHEAVEMDRAIG QAGSLTSSEDTLTVV TADHSHVFTFGGYTP RGNSIFGLAPMLSDT DKKPFTAILYGNGPG YKVVGGERENVSMVD YAHNNYQAQSAVPLR HETHGGEDVAVFSKG PMAHLLHGVHEQNYV PHVMAYAACIGANLG HCAPASSAGSLAAGP LLLALALYPLSVLF
Applications : Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Figure :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : ALPL alkaline phosphatase, liver/bone/kidney [ Homo sapiens ]
Official Symbol : ALPL
Synonyms : ALPL; alkaline phosphatase, liver/bone/kidney; HOPS; alkaline phosphatase, tissue-nonspecific isozyme; TNSALP; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme; TNAP; APTNAP; AP-TNAP; FLJ40094; FLJ93059; MGC161443; MGC167935;
Gene ID : 249
mRNA Refseq : NM_000478
Protein Refseq : NP_000469
MIM : 171760
UniProt ID : P05186

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