Active Recombinant Human AMBP protein, His&hFc-tagged

• Cat.No.: AMBP-331H
• Product Overview: Active Recombinant Human AMBP protein(P02760)(20~352 aa), fused with N-terminal His tag and C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 20-352 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Alpha-1-Microglobulin (a1M) is a small protein belonging to the superfamily of lipocalins, which is present in various body fluids such as plasma and cerebrospinal fluid. it has a role in the transportation of molecules like metals and serves as a marker for certain diseases, including kidney and liver disorders.The activity of recombinant human A1M was measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate MCA-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Trypsin was diluted to 50 ug/ml in the assay buffer and 25 ul different concentrations of recombinant human a1M (MW: 64 KD) was incubated with 25ul diluted trypsin at 37°C for 15 minutes.Loading 50 µL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 µL of 20 µM substrate.nclude a substrate blank containing 50 µL of assay buffer and 50 µL of 20 µM substrate.Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes.The result was shown and it was obvious that recombinant human a1M significantly decreased trypsin activity. The inhibition IC50 was <58 nM.
• Molecular Mass: 64.3kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: AMBP alpha-1-microglobulin/bikunin precursor [ Homo sapiens ]
• Official Symbol: AMBP
• Synonyms: AMBP; alpha-1-microglobulin/bikunin precursor; ITI, ITIL; protein AMBP; bikunin; complex forming glycoprotein heterogeneous in charge; EDC1; growth inhibiting protein 19; HCP; HI30; IATIL; inter alpha trypsin inhibitor light chain; ITILC; protein HC; trypstatin; uristatin; uronic acid rich protein; UTI; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge; A1M; ITI; ITIL
• Gene ID: 259
• mRNA Refseq: NM_001633
• Protein Refseq: NP_001624
• MIM: 176870
• UniProt ID: P02760