Recombinant Human AMELX protein, His-tagged
Cat.No. : | AMELX-12H |
Product Overview : | Recombinant Human AMELX(Met17-Asp191) fused with His tag at N-terminal was expressed in Yeast. |
- Specification
- Gene Information
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Description : | Amelogenin is the name for a series of closely related proteins involved in amelogenesis, the development of enamel. They are a type of extracellular matrix (ECM) protein, which, together with ameloblastins, enamelins, and tuftelins direct the mineralization of enamel to form a highly organized matrix of rods, interrod crystal, and protein. Amelogenins are believed to be involved in the organizing of enamel rods during tooth development. These proteins regulate the initiation and growth of hydroxyapatite crystals during the mineralization of enamel. In addition, amelogenins appear to aid in the development of cementum by directing cementoblasts to the tooth's root surface. |
Source : | Yeast |
Species : | Human |
Tag : | His |
Form : | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
AA Sequence : | MGHHHHHHGSGSLEVLFQGPMPLPP HPGHPGYINFSYEVLTPLKWYQSIR PPYPSYGYEPMGGWL HHQIIPVLSQQHPPTHTLQPHHHIP VVPAQQPVIPQQPMMPVPGQHSMTP IQHHQPNLPPPAQQP YQPQPVQPQPHQPMQPQPPVHPMQP LPPQPPLPPMFPMQPLPPMLPDLTL EAWPSTDKTKREEVD |
Endotoxin : | Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Purity : | Greater than 95% as determined by reducing SDS-PAGE. |
Storage : | Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months. |
Reconstitution : | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Gene Name : | AMELX amelogenin, X-linked [ Homo sapiens ] |
Official Symbol : | AMELX |
Synonyms : | AMELX; amelogenin, X-linked; AMG; AI1E; AIH1; ALGN; AMGL; AMGX; |
Gene ID : | 265 |
mRNA Refseq : | NM_001142 |
Protein Refseq : | NP_001133 |
MIM : | 300391 |
UniProt ID : | Q99217 |
Chromosome Location : | Xp22.31-p22.1 |
Function : | cell surface binding; growth factor activity; hydroxyapatite binding; identical protein binding; protein binding; structural constituent of tooth enamel; structural constituent of tooth enamel; |
Products Types
◆ Recombinant Protein | ||
AMELX-305R | Recombinant Rat AMELX Protein, His (Fc)-Avi-tagged | +Inquiry |
AMELX-015H | Recombinant Human AMELX Protein, (AFPn)-His-TEV-tagged | +Inquiry |
AMELX-141R | Recombinant Rhesus Macaque AMELX Protein, His (Fc)-Avi-tagged | +Inquiry |
AMELX-7077H | Recombinant Human AMELX, His-tagged | +Inquiry |
AMELX-7079H | Recombinant Human AMELX, His-tagged | +Inquiry |
◆ Lysates | ||
AMELX-70HCL | Recombinant Human AMELX cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAMELX undergoes various post-translational modifications, including phosphorylation and glycosylation, which contribute to its proper folding, secretion, and enamel matrix assembly.
AMELX gene regulation during tooth development is orchestrated by a complex interplay of transcription factors, epigenetic modifications, and signaling pathways, ensuring precise spatiotemporal expression for enamel formation.
AMELX contributes to the mechanical properties of enamel, such as its hardness, resilience, and wear resistance, through its involvement in enamel crystal organization and interlocking.
Manipulating AMELX expression or activity holds potential for enamel regeneration strategies, such as promoting ameloblast differentiation from stem cells or developing biomimetic materials that mimic the composition and structure of natural enamel for tooth repair and restoration.
Mutations or deletions in the AMELX gene result in amelogenesis imperfecta, leading to enamel defects, such as hypoplasia or hypomineralization, and compromised tooth integrity.
AMELX interacts with other enamel matrix proteins, such as amelogenin and enamelin, as well as extracellular matrix components, facilitating enamel matrix assembly, organization, and mineralization.
Signaling pathways, including Wnt, BMP, and FGF signaling, regulate AMELX expression and function, coordinating ameloblast differentiation, enamel secretion, and mineralization processes.
AMELX is highly expressed in ameloblasts, the cells responsible for enamel formation, and its role involves the synthesis and secretion of enamel matrix proteins essential for enamel structure and hardness.
AMELX plays a critical role in enamel mineralization by participating in the nucleation and growth of hydroxyapatite crystals, influencing their size, orientation, and arrangement within the enamel matrix.
AMELX expression may change during tooth aging or in dental diseases, reflecting altered enamel turnover, enamel remineralization processes, or adaptive responses to pathological conditions.
Customer Reviews (3)
Write a reviewPrecise quantification of protein expression changes in disease states.
Reduced assay background noise for improved signal-to-noise ratio.
Wide range of available detection methods for versatility.
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