Recombinant Human Aminoacylase 1, T7-tagged

• Cat.No.: ACY1-27H
• Product Overview: Recombinant Human Aminoacylase-1 produced inE.Coliis a non-glycosylated, polypeptide chain containing amino acids 1-408 and having a total molecular mass of 46.9 kda. ACY-1 contains T7 tag at N-terminus. ACY1 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: T7
• Protein Length: 1-408 a.a.
• Description: Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Formulation: Aminoacylase-1 at a concentration of 0.1 mg/ml in 10 mM Tris, pH 8.0, 0.1% Triton X-100 and 0.002% NaN3.
• Applications: • ELISA • MS • Inhibition Assays • Western Blotting.
• Stability: ACYLASE although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: ACY1 aminoacylase 1 [ Homo sapiens ]
• Synonyms: ACY1; aminoacylase 1; ACY1D; ACYLASE; aminoacylase-1;N-acyl-L-amino-acid amidohydrolase; ACY-1; EC 3.5.1.14; N-acyl-L-amino-acid amidohydrolase
• Gene ID: 95
• mRNA Refseq: NM_000666
• Protein Refseq: NP_000657
• UniProt ID: Q03154
• Chromosome Location: 3p21.1
• MIM: 104620
• Pathway: Arginine and proline metabolism; Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid; Metabolic pathways
• Function: aminoacylase activity; hydrolase activity; metal ion binding; metal ion binding; metallopeptidase activity; protein dimerization activity; zinc ion binding