Recombinant Human AMY1A protein, His-tagged

• Cat.No.: AMY1A-7027H
• Product Overview: Recombinant Human AMY1A aa. (Ala15~Leu511 (Accession # P04745)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ala15~Leu511 (Accession # P04745)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.5.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: AMY1A amylase, alpha 1A (salivary) [ Homo sapiens (human) ]
• Official Symbol: AMY1A
• Synonyms: AMY1A; amylase, alpha 1A (salivary); AMY1; alpha-amylase 1; 1,4-alpha-D-glucan glucanohydrolase 1; amylase, salivary, alpha-1A; glycogenase; salivary alpha-amylase; salivary amylase alpha 1A
• Gene ID: 276
• mRNA Refseq: NM_001008221.1
• Protein Refseq: NP_001008222.1
• UniProt ID: P04745