Recombinant Human ANGPT2, His-tagged, Biotinylated

• Cat.No.: ANGPT2-269H
• Product Overview: Human Angiopoietin-2 Protein, Biotinylated (Biotinylated ANGPT2) Tyr 19 - Phe 496, was labeled with biotin based on rhANGPT2 Protein, which was produced in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 19-496 a.a.
• Description: The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.?
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
• Molecular Mass: Biotinylated ANGPT2, fused with 6×His tag at the C-terminus, has a calculated MW of 50.1 kDa. The predicted N-terminus is Asp 68 & Tyr 19. DTT-reduced Protein migrates as 66-90 kDa due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the Biotinylated ANGPT2 by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
• Conjugation: Biotin

Gene Information

• Gene Name: ANGPT2 angiopoietin 2 [ Homo sapiens ]
• Official Symbol: ANGPT2
• Synonyms: ANGPT2; angiopoietin 2; angiopoietin-2; Ang2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a; ANG2; AGPT2
• Gene ID: 285
• mRNA Refseq: NM_001118887
• Protein Refseq: NP_001112359
• MIM: 601922
• UniProt ID: O15123
• Chromosome Location: 8p23
• Pathway: Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem; Tie2 Signaling, organism-specific biosystem
• Function: metal ion binding; protein binding; receptor binding; receptor tyrosine kinase binding