Recombinant Human ANGPTL4, His-tagged

• Cat.No.: ANGPTL4-497H
• Product Overview: Total 220 AA. MW: 25 kDa (calculated). N-Terminal His-tag, 16 extra AA (highlighted).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. The encoded protein is a serum hormone directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity and also acts as an apoptosis survival factor for vascular endothelial cells.
• Form: Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 0.05M Acetate buffer pH 4.
• Purity: >95%
• Research topic: Energy metabolism and body weight regulation.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Biologically active protein; ELISA; Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MRGSHHHHHH GMASHMGPVQ SKSPRFASWD EMNVLAHGLL QLGQGLREHA ERTRSQLSAL ERRLSACGSA CQGTEGSTDL PLAPESRVDP EVLHSLQTQL KAQNSRIQQL FHKVAQQQRH LEKQHLRIQH LQSQFGLLDH KHLDHEVAKP ARRKRLPEMA QPVDPAHNVS RLHRLPRDCQ ELFQVGERQS GLFEIQPQGS PPFLVNCKMT SDGGWTVIQR
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
• Pathways: Diabetes pathways; Fatty acid, triacylglycerol; Metabolism of lipids and lipoproteins; PPAR signaling pathway; Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha); Transcriptional Regulation of White Adipocyte Differentiation

Gene Information

• Gene Name: ANGPTL4 angiopoietin-like 4 [ Homo sapiens ]
• Official Symbol: ANGPTL4
• Synonyms: ANGPTL4; angiopoietin-related protein 4; NL2; ARP4; FIAF; PGAR; HFARP; pp1158; ANGPTL2; angiopoietin-related protein 4; angiopoietin-like protein 4; fasting-induced adipose factor; PPARG angiopoietin related protein; hepatic angiopoietin-related protein; hepatic fibrinogen/angiopoietin-related protein; peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein; PP1158; PSEC0166; UNQ171/PRO197
• Gene ID: 51129
• mRNA Refseq: NM_139314
• Protein Refseq: NP_647475
• MIM: 605910
• UniProt ID: Q9BY76
• Chromosome Location: 19p13.3
• Function: enzyme inhibitor activity; protein binding; receptor binding