| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
mIgG2aFc-Avi |
| Predicted N Terminal : |
Gln 27 |
| Form : |
Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose. |
| Bio-activity : |
Immobilized Human LILRB4, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-2.5 ug/mL. Immobilized Human LILRB2, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-1.25 ug/mL. Immobilized Human LILRA3, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-5 ug/mL. Immobilized Human LILRB5, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 20-625 ug/mL. |
| Molecular Mass : |
The protein has a calculated MW of 66.5 kDa. The protein migrates as 66 kDa and 75-95 kDa under reducing condition (SDS-PAGE) due to glycosylation. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Characteristic : |
Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
