Recombinant Human ANGPTL7 Protein, mIgG2aFc-Avi-tagged, Biotinylated

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Recombinant Human ANGPTL7 Protein, mIgG2aFc-Avi-tagged, Biotinylated

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Cat.No. : ANGPTL7-162H
Product Overview : Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Gln 27 - Pro 346 (Accession # O43827-1). This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag.
Source : HEK293
Species : Human
Tag : mIgG2aFc-Avi
Predicted N Terminal : Gln 27
Form : Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity : Immobilized Human LILRB4, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-2.5 ug/mL. Immobilized Human LILRB2, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-1.25 ug/mL. Immobilized Human LILRA3, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-5 ug/mL. Immobilized Human LILRB5, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 20-625 ug/mL.
Molecular Mass : The protein has a calculated MW of 66.5 kDa. The protein migrates as 66 kDa and 75-95 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : ANGPTL7 angiopoietin-like 7 [ Homo sapiens ]
Official Symbol : ANGPTL7
Synonyms : ANGPTL7; angiopoietin-like 7; angiopoietin-related protein 7; AngX; CDT6; angiopoietin-like protein 7; angiopoietin-like factor (CDT6); cornea-derived transcript 6 protein; dJ647M16.1; RP4-647M16.2;
Gene ID : 10218
mRNA Refseq : NM_021146
Protein Refseq : NP_066969
UniProt ID : O43827
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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