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Recombinant Human ANGPTL7 Protein, mIgG2aFc-Avi-tagged, Biotinylated

Cat.No.: ANGPTL7-162H
Product Overview: Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Gln 27 - Pro 346 (Accession # O43827-1). This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag.
Source: HEK293
Species: Human
Tag : mIgG2aFc-Avi
Predicted N Terminal: Gln 27
Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity: Immobilized Human LILRB4, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-2.5 ug/mL. Immobilized Human LILRB2, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-1.25 ug/mL. Immobilized Human LILRA3, Fc Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 0.078-5 ug/mL. Immobilized Human LILRB5, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag with a linear range of 20-625 ug/mL.
Molecular Mass: The protein has a calculated MW of 66.5 kDa. The protein migrates as 66 kDa and 75-95 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90% as determined by SDS-PAGE.
Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: ANGPTL7 angiopoietin-like 7 [ Homo sapiens ]
Official Symbol: ANGPTL7
Synonyms: ANGPTL7; angiopoietin-like 7; angiopoietin-related protein 7; AngX; CDT6; angiopoietin-like protein 7; angiopoietin-like factor (CDT6); cornea-derived transcript 6 protein; dJ647M16.1; RP4-647M16.2;
Gene ID: 10218
mRNA Refseq: NM_021146
Protein Refseq: NP_066969
UniProt ID: O43827

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