Active Recombinant Human ANPEP protein, LFc-tagged, Low Endotoxin

• Cat.No.: ANPEP-2480H
• Product Overview: Recombinant Human ANPEP protein(P15144)(Lys69-Lys967), fused with C-terminal LFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: lFc
• Protein Length: 69-967 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >1500 pmol/min/µg.
• Molecular Mass: The protein has a predicted MW of 130.03 kDa. Due to glycosylation, the protein migrates to 140-160 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ANPEP alanyl (membrane) aminopeptidase [ Homo sapiens ]
• Official Symbol: ANPEP
• Synonyms: ANPEP; alanyl (membrane) aminopeptidase; CD13, PEPN; aminopeptidase N; aminopeptidase M; gp150; LAP1; microsomal aminopeptidase; p150; AP-M; AP-N; hAPN; alanyl aminopeptidase; myeloid plasma membrane glycoprotein CD13; APN; CD13; P150; PEPN; GP150
• Gene ID: 290
• mRNA Refseq: NM_001150
• Protein Refseq: NP_001141
• MIM: 151530
• UniProt ID: P15144