Recombinant Human ANXA6 Protein, His-tagged

• Cat.No.: ANXA6-001H
• Product Overview: Recombinant Human ANXA6 Protein 1-673 aa (full-length) with a 6xHis tag was expressed in E.coli.

Specification

• Description: Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 78.6 kDa
• Purity: >95% confirmed by 10% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-6xHis (WB).
• Applications: ELISA, WB, Antibody Production, Protein array
• Quality Control Test: 10% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-6xHis (WB) serum and peptide fingerprinting by MALDI-TOF-TOF mass spectrometry.
• Storage: Store working aliquots at 4 centigrade for up to one week.
  For extended storage, conserve at −80 centigrade.
• Expiry: 12 months from the date of purchase, if it is properly stored.
• Storage Buffer: Phosphate-Buffered Saline (PBS), pH 7.4. No preservatives added.
• Shipping: Dry Ice
• Protein length: 1-673

Gene Information

• Gene Name: ANXA6 annexin A6 [ Homo sapiens (human) ]
• Official Symbol: ANXA6
• Synonyms: ANXA6; annexin A6; p68; p70; ANX6; CBP68; CPB-II; annexin A6; 67 kDa calelectrin; annexin VI (p68); annexin-6; calcium-binding protein p68; calelectrin; calphobindin II; chromobindin-20; lipocortin VI; testis secretory sperm-binding protein Li 198a
• Gene ID: 309
• mRNA Refseq: NM_001155
• Protein Refseq: NP_001146
• MIM: 114070
• UniProt ID: P08133

Reviews

02/19/2022

the manufacturer's support is crucial for researchers using ANXA6 protein in trials.

02/01/2020

These resources can enhance the efficiency and accuracy of experimental workflows and contribute to successful outcomes.

12/29/2019

They may offer educational resources such as technical guides, protocols, and application notes, which can aid researchers in understanding the principles, techniques, and potential applications of ANXA6 protein in their studies.

06/21/2017

Their collaboration, expertise, and customized solutions contribute to the successful execution of experiments, reliable data generation, and the advancement of scientific knowledge in the field.

Q&As

How is ANXA6 associated with cardiovascular diseases?

ANXA6 has been implicated in various cardiovascular diseases such as atherosclerosis, myocardial infarction, and heart failure. It has been suggested that altered ANXA6 expression and function may contribute to abnormal lipid metabolism, inflammation, and vascular calcification, which are hallmarks of cardiovascular pathologies.

How is ANXA6 involved in membrane trafficking?

ANXA6 interacts with intracellular membranes and can play a role in regulating membrane trafficking processes such as endocytosis, exocytosis, and membrane repair. It has been shown to influence the fusion and fission events necessary for proper membrane trafficking.

Is ANXA6 involved in autophagy?

Yes, ANXA6 has been implicated in autophagy, a cellular process responsible for the degradation and recycling of cellular components. It has been suggested that ANXA6 may play a role in autophagosome formation and maturation by interacting with proteins involved in autophagy regulation.

Are there any pharmacological agents that can modulate ANXA6 expression or activity?

Some studies have identified pharmacological agents that can modulate ANXA6 expression or activity. For example, certain drugs have been reported to regulate ANXA6 expression levels in cancer cells. Additionally, compounds affecting calcium signaling or phospholipid metabolism can indirectly influence ANXA6 function. However, more research is needed to identify specific pharmacological agents that can selectively target ANXA6.

Can ANXA6 be targeted for therapeutic interventions?

Modulating ANXA6 expression or function may have potential therapeutic value, especially in diseases where ANXA6 plays a critical role. Developing drugs that selectively target ANXA6 or its interacting partners could potentially lead to novel therapeutic strategies. However, further research is needed to understand the precise mechanisms and validate its therapeutic potential.

Does ANXA6 have any specific binding partners?

ANXA6 can interact with several proteins and cellular components. It has been reported to interact with various phospholipids, membrane proteins, cytoskeletal proteins, and signaling molecules. Some of its known binding partners include clathrin, caveolin, Rab GTPases, and integrins.

What are the future directions of research on ANXA6?

Future research on ANXA6 may focus on elucidating its precise molecular mechanisms and functional roles in various cellular processes. Further studies are also needed to understand its involvement in specific diseases to develop targeted therapies and exploit its potential as a diagnostic or prognostic marker.

Can ANXA6 be used as a diagnostic or prognostic marker?

ANXA6 has been investigated as a potential biomarker in several diseases. Its altered expression and subcellular localization in cancer tissues have shown promise for its diagnostic and prognostic value. However, further research is needed to validate its clinical utility in different diseases.

Are there any known therapeutic strategies targeting ANXA6?

Currently, there are no specific therapeutic strategies directly targeting ANXA6. However, since ANXA6 plays a role in various diseases, understanding its mechanisms of action may facilitate the development of targeted therapies. Some studies have explored the use of small molecules or inhibitors that indirectly modulate ANXA6 function or its interacting partners as potential therapeutic interventions.

Are there any specific drugs targeting ANXA6?

Currently, there are no specific drugs targeting ANXA6. However, studies exploring the potential therapeutic value of modulating ANXA6 expression and activity are ongoing, especially in the context of cancer and other diseases where ANXA6 is implicated.

Can ANXA6 interact with other proteins or molecules?

Yes, ANXA6 can interact with various proteins and molecules. It has been shown to interact with membrane lipids, calcium ions, and other annexin family members. ANXA6 also interacts with proteins involved in intracellular trafficking, such as dynamin, clathrin, and various Rab GTPases. Additionally, ANXA6 can interact with signaling molecules, cytoskeletal proteins, and membrane receptors, suggesting its involvement in diverse cellular processes.

Are there any known genetic mutations or polymorphisms in ANXA6?

Certain genetic mutations and polymorphisms have been identified in ANXA6. For example, in some cancers, alterations in ANXA6 expression levels and mutations affecting its function have been observed. The significance of other genetic variations in ANXA6 and their association with disease phenotypes require further investigation.

How is ANXA6 involved in cellular signaling?

ANXA6 can function as a scaffold protein and participate in diverse signaling pathways. It can interact with signaling molecules like protein kinases, phospholipases, and second messengers, modulating their activities and contributing to cellular signaling events, including calcium signaling and receptor trafficking.

Are there any genetic mutations or polymorphisms in ANXA6 associated with diseases?

While ANXA6 mutations or polymorphisms have not been extensively studied, some studies have reported genetic variants in ANXA6 that are associated with certain diseases. For example, specific ANXA6 polymorphisms have been linked to the risk of developing Parkinson's disease and schizophrenia. However, more research is needed to investigate the association between ANXA6 genetic variations and diseases.

Can ANXA6 be used as a biomarker for diseases?

ANXA6 has been investigated as a potential biomarker for several diseases. Its expression levels and localization have been analyzed in various conditions, including cancer, cardiovascular diseases, and neurodegenerative disorders. However, further studies are needed to establish its utility as a diagnostic or prognostic biomarker in clinical settings.

Can ANXA6 be used as a drug delivery vehicle?

As a membrane-binding protein, ANXA6 has been explored for drug delivery applications. Its ability to interact with intracellular membranes and regulate membrane trafficking processes makes it a potential candidate for targeted drug delivery. However, more research is needed to evaluate its efficacy and safety in drug delivery systems.

Does ANXA6 have any role in immune responses?

Yes, ANXA6 has been reported to have immunomodulatory properties. It can regulate immune cell migration, phagocytosis, and cytokine release. ANXA6 has also been implicated in autoimmune diseases, such as systemic lupus erythematosus, suggesting its involvement in immune dysregulation.

What are the potential roles of ANXA6 in disease?

ANXA6 has been linked to several pathological conditions. It has been associated with cancers, where its altered expression and subcellular localization have been observed. ANXA6 has also been implicated in metabolic disorders, cardiovascular diseases, and neurodegenerative diseases like Alzheimer's and Parkinson's.

Does ANXA6 have any role in insulin resistance or diabetes?

ANXA6 has been implicated in metabolic disorders like insulin resistance and diabetes. It has been shown to influence insulin signaling and glucose homeostasis in animal models. Altered ANXA6 expression and localization have been observed in insulin-resistant tissues, suggesting its involvement in insulin sensitivity and glucose metabolism.

Are there any animal models available to study ANXA6 function and its role in diseases?

Yes, animal models, such as genetically modified mice, have been used to study ANXA6 function and its involvement in disease processes. Knockout or transgenic animal models with altered ANXA6 expression allow researchers to investigate its specific roles in various systems and diseases.

Can ANXA6 play a role in cancer progression?

Yes, ANXA6 has been implicated in cancer progression. Altered expression levels of ANXA6 have been observed in different types of cancer, and it has been associated with tumor growth, invasion, metastasis, and drug resistance. ANXA6 can modulate various cellular processes in cancer cells, including cell proliferation, migration, and angiogenesis, making it a potential therapeutic target in cancer treatment.