Recombinant Human AP2B1 293 Cell Lysate

• Cat.No.: AP2B1-8815HCL

Specification

• Description: Antigen standard for adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AP2B1 adaptor-related protein complex 2, beta 1 subunit [ Homo sapiens ]
• Official Symbol: AP2B1
• Synonyms: AP2B1; adaptor-related protein complex 2, beta 1 subunit; ADTB2, CLAPB1; AP-2 complex subunit beta; beta-adaptin; beta-2-adaptin; adaptin, beta 2 (beta); adaptor protein complex AP-2 subunit beta; adapter-related protein complex 2 beta subunit; clathrin assembly protein complex 2 beta large chain; plasma membrane adaptor HA2/AP2 adaptin beta subunit; clathrin-associated/assembly/adaptor protein, large, beta 1; ADTB2; AP105B; CLAPB1; AP2-BETA; DKFZp781K0743
• Gene ID: 163
• mRNA Refseq: NM_001030006
• Protein Refseq: NP_001025177
• MIM: 601025
• UniProt ID: P63010
• Chromosome Location: 17q11.2-q12
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Disease, organism-specific biosystem; EGFR downregulation, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, conserved biosystem; Endocytosis, organism-specific biosystem
• Function: binding; clathrin binding; protein binding; protein transporter activity