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Recombinant Human AP2M1 Protein (1-435 aa), His-tagged

Cat.No. : AP2M1-1328H
Product Overview : Recombinant Human AP2M1 Protein (1-435 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Transport. Protein Description: Full Length.
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Description : Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different mbrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to mbrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of mbranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the mbrane proteins involved in receptor-mediated endocytosis. AP-2 ses to play a role in the recycling of synaptic vesicle mbranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmbrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmbrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in mbrane-associated AP-2. The mbrane-specific phosphorylation event appears to involve assbled clathrin which activates the AP-2 mu kinase AAK1 . Plays a role in endocytosis of frizzled family mbers upon Wnt signaling.
Source : Yeast
Species : Human
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 51.7 kDa
Protein length : 1-435 aa
AA Sequence : MIGGLFIYNHKGEVLISRVYRDDIG RNAVDAFRVNVIHARQQVRSPVTNI ARTSFFHVKRSNIWLAAVTKQNVNA AMVFEFLYKMCDVMAAYFGKISEEN IKNNFVLIYELLDEILDFGYPQNSE TGALKTFITQQGIKSQHQTKEEQSQ ITSQVTGQIGWRREGIKYRRNELFL DVLESVNLLMSPQGQVLSAHVSGRV VMKSYLSGMPECKFGMNDKIVIEKQ GKGTADETSKSGKQSIAIDDCTFHQ CVRLSKFDSERSISFIPPDGEFELM RYRTTKDIILPFRVIPLVREVGRTK LEVKVVIKSNFKPSLLAQKIEVRIP TPLNTSGVQVICMKGKAKYKASENA IVWKIKRMAGMKESQISAEIELLPT NDKKKWARPPISMNFEVPFAPSGLK VRYLKVFEPKLNYSDHDVIKWVRYI GRSGIYETRC
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Gene Name : AP2M1 adaptor-related protein complex 2, mu 1 subunit [ Homo sapiens ]
Official Symbol : AP2M1
Synonyms : AP2M1; CLAPM1; AP50; mu subunit; medium 1;
Gene ID : 1173
mRNA Refseq : NM_001025205
Protein Refseq : NP_001020376
MIM : 601024
UniProt ID : Q96CW1

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (20)

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What are the applications of AP2M1 in scientific research? 12/05/2022

AP2M1 can be used in scientific research to study the regulation mechanism of intracellular protein and the pathogenesis of disease.

What are the mechanisms of recombinant AP2M1 in cancer therapy? 06/13/2022

AP2M1 can interfere with tumor cell signaling pathways, thereby inhibiting tumor growth and spread.

What are the remaining challenges in the research of AP2M1 ? 05/23/2022

AP2M1 research still needs to address safety, preparation methods and dosage challenges.

Can AP2M1 be used in personalized medicine? 05/15/2022

The personalized medicine is the development trend in the future, and we can expect the application prospect of AP2M1 in this field.

Does AP2M1 have side effects? 09/20/2021

The side effects of recombinant AP2M1 are not known and further studies are needed to evaluate its safety.

Can AP2M1 be applied through gene therapy? 06/07/2021

It is theoretically possible to apply recombinant AP2M1 with gene therapy, but more research is needed to confirm this.

What animal model can be used for the study of AP2M1 ? 06/04/2021

Mouse models are currently available to further understand the pharmacological properties of AP2M1 .

Can AP2M1 be administered orally? 12/21/2020

There are no results of studies on oral administration, but attempts to develop the pathway could be made.

What are the advantages of AP2M1 compared with drug therapy? 08/10/2020

AP2M1 can play a therapeutic role by interfering with intracellular signaling pathways, sometimes avoiding adverse drug reactions.

What is the dosage and use of AP2M1 ? 06/28/2020

The current dosage and mode of use are still under investigation, and further clinical trials are needed to determine the best use.

What is the future development direction of AP2M1 research? 03/10/2020

Future studies could focus on optimizing the preparation method, study dose and safety, and further evaluate the clinical prospects of AP2M1 .

How does AP2M1 act on human cells? 03/06/2020

AP2M1 can bind to specific receptors in human cells to regulate signaling pathways.

Can recombinant AP2M1 be used for drug delivery? 02/27/2020

AP2M1 may be used as part of the drug delivery system to achieve targeted drug delivery by regulating intracellular delivery mechanisms.

Does AP2M1 interact with other proteins? 12/02/2019

Yes, the recombinant AP2M1 protein can interact with other proteins to affect cell function.

How is the research progress of AP2M1 ? 11/10/2019

AP2M1 research is still in its infancy, but there have been some promising discoveries.

What is the preparation method of AP2M1 ? 09/30/2019

AP2M1 is generally expressed and purified in cells by genetic engineering technology.

What is the stability of AP2M1 ? 07/19/2019

The stability of AP2M1 may be affected by a variety of factors and needs to be optimized through further research.

Does AP2M1 have antiviral activity? 04/28/2019

AP2M1 may have antiviral activity, but further validation and research is needed.

Can recombinant AP2M1 be used to treat clinical diseases? 03/28/2019

It is not currently in clinical use, but one can expect its potential in treating diseases such as cancer.

What are the advantages of AP2M1 in treatment? 02/26/2019

AP2M1 has high specificity and selectivity, and can be targeted for treatment.

Customer Reviews (4)

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Reviews
02/24/2022

    The reproducibility of AP2M1 is very good, and the results remain consistent even after repeated experiments, which makes me more confident in the experimental results.

    04/05/2021

      The short half-life and high clearance characteristics of AP2M1 enable it to reach the therapeutic concentration quickly and maintain a stable blood concentration, thus improving the therapeutic effect.

      12/14/2019

        In the case of adding different chemicals or drugs, the stability of this protein is still very good, indicating that it has excellent anti-interference ability and stability.

        11/24/2019

          AP2M1 in Western blot is very good, even if it is stored for a long time, it will not affect its performance.

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