Recombinant Human APAF1 Protein (M1-S97), His tagged

• Cat.No.: APAF1-0784H
• Product Overview: Recombinant Human 6His-TEV-(G)-CASP9(M1-S97) Protein was expressed in E. coli.

Specification

• Description: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Protein length: M1-S97
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: APAF1 apoptotic peptidase activating factor 1 [ Homo sapiens (human) ]
• Official Symbol: APAF1
• Synonyms: APAF1; apoptotic peptidase activating factor 1; apoptotic peptidase activating factor, apoptotic protease activating factor; apoptotic protease-activating factor 1; APAF 1; CED4; APAF-1; DKFZp781B1145
• Gene ID: 317
• mRNA Refseq: NM_001160
• Protein Refseq: NP_001151
• MIM: 602233
• UniProt ID: O14727

Reviews

11/11/2021

This protein is really excellent, every time it is used, it maintains a very stable performance, which can be seen that its production process is very careful.

10/19/2021

Short half-life and high clearance properties of AP5Z1 make it an even greater advantage in emergency situations where rapid therapeutic effect is needed.

04/25/2021

Green production process of AP5Z1 is very environmentally friendly, from the selection of raw materials to the production process reflects the concept of green production, without any negative impact on the environment.

02/13/2019

The repeatability of AP5Z1 can be said to be unmatched, and the results are very consistent in every experiment or application, which is an important reflection of its excellent quality.

Q&As

What is the biological function of APAF1 ?

Recombinant APAF1 protein is an important apoptosis-promoting factor that can play a key role in programmed apoptosis.

Are there any side effects of APAF1 ?

APAF1 is almost non-toxic side effects, will not cause great harm to the human body.

Can APAF1 replace existing antitumor drugs?

Recombinant APAF1 protein cannot completely replace existing anti-tumor drugs, but it can be used as an important part of comprehensive tumor therapy, which plays an important role in improving the therapeutic effect and reducing the toxic side effects of dose.

What is the latest progress in the clinical application of APAF1 ?

The latest research shows that the APAF1 can be used as a target of novel tumor immunotherapy, and has a good effect on tumor treatment.

Does recombinant APAF1 have a role in human ER stress response?

Recombinant APAF1 protein plays an important role in human endoplasmic reticulum stress response and can protect the maintenance of endoplasmic reticulum function by inhibiting apoptosis.

How to prepare APAF1 ?

APAF1 can be prepared by gene recombination technology, which first expresses the target protein in a large number of expression hosts such as bacteria, and then obtains high purity APAF1 through purification technology.

What is the expression pattern of APAF1 in the human body?

APAF1 is widely distributed in the human body, expressed not only in tumor cells, but also in many tissues such as heart, liver, and stomach.

What clinical tests and treatments can APAF1 be used for?

APAF1 can be used in early diagnosis of tumor, prognosis of tumor, and monitoring of tumor therapeutic effect.

Can APAF1 be clearly seen in electron microscope photos?

The APAF1 has a clear recognition ability for electron microscopy photos, and can be used to observe the effect of specific cells and gene transformations.

What is the relationship between APAF1 and p53 protein?

APAF1 and p53 are closely related in the activation and regulation of apoptosis pathway.

Can small molecule compounds regulate the expression and activity of APAF1?

Yes, small molecule compounds have regulatory effects on the expression and activity of APAF1.

What is the antigenicity of APAF1 ?

APAF1 is low in antigenicity, so it is not easy to produce an immune response.

What is apoptosis resistance? Does APAF1 affect apoptosis resistance?

Apoptosis resistance is a physical state in which tumor cells overcome the production of apoptotic signals. APAF1 can regulate apoptosis pathway and help to reduce apoptosis resistance.

Does APAF1 have application value in drug dose adjustment?

Yes, the expression and activity level of APAF1 can be used as a standard for drug dose adjustment, which can play a regulatory role in the effect of tumor therapy.

What is the relationship between APAF1 and liver cancer and breast cancer?

Recombinant APAF1 protein plays an important role in the development of liver cancer and breast cancer, and can be used as a target for early diagnosis and treatment.

What is the role of APAF1 in tumor resistance?

APAF1 in tumor resistance may be affected by some factors that can lead to the weakening of apoptotic pathway processes, thus affecting the efficacy of tumor therapy.

Can APAF1 be used to study the relationship between apoptosis and tumor?

Yes, the recombinant APAF1 protein can be used to study the role and mechanism of apoptosis signaling pathway in tumorigenesis and development.

Which experimental kits can APAF1 be used to prepare?

APAF1 can be used in Western blot kits, ELISA kits, Western blot kits, etc.

Does APAF1 have potential applications in tumor therapy?

Yes, recombinant APAF1 protein in tumor therapy can be used to develop novel targeted therapeutic drugs, such as vaccines and immune checkpoint inhibitors.

What is the position and role of APAF1 in apoptosis pathway?

PAF1 can bind to other apoptotic proteins such as cytochrome c to promote the activation of Karber gene and initiate the apoptotic mechanism.