Recombinant Human APLN protein, His & MBP-tagged

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Recombinant Human APLN protein, His & MBP-tagged

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Cat.No. : APLN-8262H
Product Overview : Recombinant Human APLN aa. (Ser24~Phe77) fused with N-terminal His & MBP tag was produced in E. coli cells.
Description : This gene encodes a peptide that functions as an endogenous ligand for the G-protein coupled apelin receptor. The encoded preproprotein is proteolytically processed into biologically active C-terminal peptide fragments. These peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1.
Source : E. coli
Species : Human
Tag : His & MBP
Form : Freeze-dried powder
Molecular Mass : 56kDa as determined by SDS-PAGE reducing conditions.
Protein length : Ser24~Phe77
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 11.9.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage Buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
SDS-PAGE :

Gene Name : APLN apelin [ Homo sapiens (human) ]
Official Symbol : APLN
Synonyms : APLN; apelin; APEL; XNPEP2; AGTRL1 ligand; APJ endogenous ligand
Gene ID : 8862
mRNA Refseq : NM_017413.4
Protein Refseq : NP_059109.3
UniProt ID : Q9ULZ1
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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