Recombinant Human APOA2 protein, His-tagged

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Recombinant Human APOA2 protein, His-tagged

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Cat.No. : APOA2-357H
Product Overview : Recombinant Human APOA2 protein (Ala19-Gln100), fused to His tag at C-terminus, was expressed in E.coli.
Description : Apolipoprotein A-II(APO-A2) stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. It is produced by the liver and is involved in cholesteryl ester formation and cholesterol transport from tissues to the liver. Recent studies have established the interaction between APOA2 -256T>C polymorphism and dietary saturated fatty acids intake in relation to obesity on healthy individuals. Significant interaction between APOA2-saturated fatty acids intake and body mass index (BMI). The result of this study confirmed the interaction between APOA2 -256T>C polymorphism and SFAs intake with BMI in type 2 diabetic patients.
Source : E.coli
Species : Human
Tag : His
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 11.2 kDa. The protein migrates as 11 kDa and 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : 82
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of APOA2-357H :

Human Apolipoprotein A-II, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Gene Name : APOA2
Official Symbol : APOA2
Synonyms : APOA2; apolipoprotein A-II; apolipoprotein A2; apoAII; Apo-AII; ApoA-II
Gene ID : 336
mRNA Refseq : NM_001643
Protein Refseq : NP_001634
MIM : 107670
UniProt ID : P02652

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