Recombinant Human APOE4 protein, His-Trx-tagged, Amine-Labeled Biotinylated

• Cat.No.: APOE4-2869HB
• Product Overview: Biotinylated Recombinant Human SEZ6 protein(Q53EL9-1)(Leu20-His925) is expressed from HEK293 with His tag and Avi tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Leu20-His925
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human SEZ6, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 100.7 kDa. Due to glycosylation, the protein migrates to 170-200 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt; -80°C for 3-6 months after reconstitution; 2-8°C for 2-7 days after reconstitution; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Conjugation: Biotin

Gene Information

• Gene Name: SEZ6 seizure related 6 homolog (mouse) [ Homo sapiens ]
• Official Symbol: SEZ6
• Gene ID: 124925
• mRNA Refseq: NM_178860.4
• Protein Refseq: NP_849191.3
• UniProt ID: Q53EL9