Recombinant Human APP Protein, Fc-tagged

• Cat.No.: APP-62H
• Product Overview: Recombinant Human Amyloid Precursor is produced by our Mammalian expression system and the target gene encoding Leu18-Lys612 is expressed with a Fc tag at the C-terminus.

Specification

• Description: Amyloid precursor protein (APP) is a type I membrane protein with several isoforms due to alternative splicing, performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Of the three major splice isoforms of APP (APP695, APP751, and APP770) APP695 is the predominant neuronal form from which Amyloid beta peptide and transcriptionally-active cleaved intracellular domain of APP (AICD) are preferentially generated by selective processing through the amyloidogenic pathway. Human APP695 consists of a 17 amino acid (aa) signal sequence, a 607 aa extracellular domain (ECD), a 24 aa transmembrane domain, and a 47 aa cytoplasmic domain. Within the ECD, human APP695 shares 97% aa sequence identity with mouse and rat APP695. Amyloid beta is a major molecule implicated in pathogenesis of Alzheimer's disease (AD) and related dementias. AICD regulates expression by direct promoter binding of multiple genes, including APP itself, the beta-secretase, BACE-1 and the Amyloid beta-degrading enzyme, Neprilysin. As such, APP695 plays an important role in brain development, learning and memory, synaptic plasticity, and neurodegeneration including AD.
• Source: Human Cells
• Species: Human
• Tag: Fc
• Form: Lyophilized from a 0.2 um filtered solution of PBS, 5% trehalose, pH7.4.
• Molecular Mass: Predicted Molecular Weight: 94.6kDa
  Apparent Molecular Weight: 120-145kDa, reducing conditions.
• AA Sequence: Leu18-Lys612
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: APP amyloid beta precursor protein [ Homo sapiens (human) ]
• Official Symbol: APP
• Synonyms: Amyloid Precursor; Amyloid Precursor Protein 695; APP695; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma; alpha-sAPP
• Gene ID: 351
• mRNA Refseq: NM_000484.4
• Protein Refseq: NP_000475.1
• MIM: 104760
• UniProt ID: P05067