Recombinant Human ARTN Protein

• Cat.No.: ARTN-703H
• Product Overview: Recombinant Human ARTN Protein is a single non-glycosylated polypeptide chain containing 113 amino acids without tag purified from serum-free CHO growth media by ion-exchange chromatography and gel-filtration. Protein is sterile-filtered through 0.22 µm filter.

Specification

• Description: This gene encodes a secreted ligand of the glial cell line-derived neurotrophic factor (GDNF) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein signals through the RET receptor and GFR alpha 3 coreceptor, and supports the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. This protein has also been shown to promote tumor growth, metastasis, and drug resistance in mammary carcinoma.
• Source: CHO
• Species: Human
• Form: Homodimer
• Molecular Mass: 11.9 kDa
• Endotoxin: Less than 1 EU/mg of protein as determined by LAL method
• Applications: Product is suitable for cell culture and in vivo testing. Protein is biologically active. Biological activity of purified artemin was tested in in vitro receptor-dependent cellular morphology change induction assay using U2OS cells expressing Ret and GFRα-3 receptor complex
• Quality Control Test: SDS-PAGE and Western-Blot analysis
• Storage: Store at -70 centigrade upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.
• Concentration: 1 mg/mL. Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
• Storage Buffer: 10 mM Na-citrate; 150 mM NaCl pH 4.5
• Shipping: Shipped in dry-ice

Gene Information

• Gene Name: ARTN artemin [ Homo sapiens (human) ]
• Official Symbol: ARTN
• Synonyms: ARTN; artemin; ART; EVN; NBN; ENOVIN; artemin; neublastin
• Gene ID: 9048
• mRNA Refseq: NM_057090
• Protein Refseq: NP_476431
• MIM: 603886
• UniProt ID: Q5T4W7