Recombinant Human ASAH2 Cell Lysate, Myc/DDK-tagged

• Cat.No.: ASAH2-365HCL
• Product Overview: Transient overexpression lysate of N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 with a C-Myc/DDK tag was expressed in HEK293T.
  Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5 μg TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25 mM Tris-HCl pH7.6, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20 centigrade before shipping.
  Components:
  1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 μg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250 μL 2×SDS Sample Buffer (4% SDS, 125 mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100 mM DTT)

Specification

• Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 83.1 kDa
• Stability: Avoid repeated freeze-thaw cycles.
• Storage: Upon receiving, store the sample at -80 centigrade. Also after dilution, the protein sample should be aliquoted and stored at -80 centigrade for long term storage. Lysate samples are stable for 12 months from the date of receipt when stored at -80 centigrade.
• Reconstitution: Lysate samples can be diluted with 2×SDS Sample Buffer provided.
• Shipping: Dry ice

Gene Information

• Gene Name: ASAH2 N-acylsphingosine amidohydrolase 2 [ Homo sapiens (human) ]
• Official Symbol: ASAH2 N-acylsphingosine amidohydrolase 2 [ Homo sapiens (human) ]
• Synonyms: ASAH2; N-acylsphingosine amidohydrolase 2; HNAC1; BCDase; LCDase; NCDase; N-CDase; neutral ceramidase; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; acylsphingosine deacylase 2; mitochondrial ceramidase; neutral/alkaline ceramidase; non-lysosomal ceramidase; EC 3.5.1.23
• Gene ID: 56624
• mRNA Refseq: NM_019893
• Protein Refseq: NP_063946
• MIM: 611202
• UniProt ID: Q9NR71