Active Recombinant Human ASAH2, His-tagged

• Cat.No.: ASAH2-284H
• Product Overview: Recombinant Human ASAH2 (Accession # AAF86240) Thr15-Ile761, fused with an N-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 15-761 a.a.
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in MES, NaCl and Sodium Cholate.
• Bio-activity: Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid. The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human ASAH2, His-tagged has a calculated MW of 83 kDa. In SDS-PAGE migrates as 132 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 [ Homo sapiens ]
• Official Symbol: ASAH2
• Synonyms: ASAH2; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; neutral ceramidase; mitochondrial ceramidase; non-lysosomal ceramidase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; N-acylsphingosine amidohydrolase 2; HNAC1; BCDase; LCDase; NCDase; N-CDase
• Gene ID: 56624
• mRNA Refseq: NM_001143974
• Protein Refseq: NP_001137446
• MIM: 611202
• UniProt ID: Q9NR71
• Chromosome Location: 10q11.23