Recombinant Human ASAM protein, His-tagged

• Cat.No.: ASAM-4022H
• Product Overview: Recombinant Human ASAM protein(22-229 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 22-229 aa
• AA Sequence: EIKRVAEEKVTLPCHHQLGLPEKDT LDIEWLLTDNEGNQKVVITYSSRHV YNNLTEEQKGRVAFASNFLAGDASL QIEPLKPSDEGRYTCKVKNSGRYVW SHVILKVLVRPSKPKCELEGELTEG SDLTLQCESSSGTEPIVYYWQRIRE KEGEDERLPPKSRIDYNHPGRVLLQ NLTMSYSGLYQCTAGNEAGKESCVV RVTVQYVQ
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Reviews

08/31/2020

It has consistently demonstrated high sensitivity and accuracy in detecting and quantifying target molecules, allowing for precise and reliable measurement of analytes in my experimental samples.

03/12/2020

Its versatility and reliability make it a top choice for researchers in various fields.

10/16/2017

The ASAM Protein's adaptability has significantly expanded the scope of my investigations and broadened the possibilities for my research.

Q&As

Are there any known genetic variations or polymorphisms in ASAM?

The presence of genetic variations or polymorphisms in ASAM has not been extensively investigated, and further research is required to ascertain this.

Does ASAM affect the opening or closing of MiniK channels?

ASAM primarily affects the opening of MiniK channels, regulating their overall activity and the efflux of potassium ions.

How does ASAM modulate sodium and MiniK channels?

ASAM binds to sodium and MiniK channels, altering their activity and influencing the flow of ions through these channels.

Is ASAM associated with any specific diseases?

Currently, there are no reported disease associations specific to ASAM. More research is needed to determine its potential involvement in diseases.

Is ASAM expressed in all cell types?

ASAM is not universally expressed in all cell types, and its expression pattern may vary depending on the tissue or cell type.

What are the cellular consequences of ASAM's modulation of sodium and MiniK channels?

ASAM's modulation of these channels can impact the excitability and electrical properties of cells, influencing their behavior and function.

Does ASAM affect the opening or closing of sodium channels?

ASAM can affect both the opening and closing of sodium channels, leading to changes in their overall activity.