Recombinant Human ASCL1 Protein, His-tagged

• Cat.No.: ASCL1-23H
• Product Overview: Recombinant Human ASCL1 Protein(NP_004307.2)(Lys84~Phe236), fused with N-terminal His Tag, was expressed in E. coli.

Specification

• Description: This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Lys84~Phe236
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein.
• Notes: The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
• Storage: Avoid repeated freeze/thaw cycles.
  Store at 2-8°C for one month.
  Aliquot and store at -80ºC for 12 months.
• Concentration: 200 µg/mL
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: ASCL1 achaete-scute family bHLH transcription factor 1 [ Homo sapiens (human) ]
• Official Symbol: ASCL1
• Synonyms: ASH1; HASH1; MASH1; bHLHa46
• Gene ID: 429
• mRNA Refseq: NM_004316.4
• Protein Refseq: NP_004307.2
• MIM: 100790
• UniProt ID: P50553