Recombinant Human ASH1L protein, GST-tagged

• Cat.No.: ASH1L-47H
• Product Overview: Recombinant Human ASH1L protein, fused with N-terminal GST tag was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 2022-2302
• Description: ASH1L is a large , multi-domain protein associated with actively transcribed regions of chromatin, ASH1L is the human homolog of Drosophila Ash1, a Trithorax group protein. Like its counterpart in Drosophila, ASH1L contains a SET histone methyltransferase domain and has been found to play a role in the regulation of Hox gene expression. Although the ASH1L SET domain has been shown in vitro to methylate histone peptides on lysine-4 of histone H3 (H3K4), ASH1L is an H3K36 methyltransferase both in vivo and in vitro with nucleosomes as substrate. Recruitment of ASH1L by the ncRNA DBE-T to the chromosome 4q35 locus associated with FSHD (facioscapulohumeral muscular dystrophy) leads to increased H3K36me2 and inappropriate gene derepression at the FSHD locus. In a possible positive feedback loop, ASH1L increases expression of DBE-T itself, suggesting ASH1L’s methyltransferase activity or its interaction with DBE-T as potential therapeutic targets for FSHD.
• Form: Solution of purified recombinant protein in 50 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1 mM TCEP, 10% (v/v) glycerol
• Molecular Mass: 59.5 kDa
• Storage: Store at -80centigrade. Thaw quickly and store on ice before use. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ASH1L ash1 (absent, small, or homeotic)-like (Drosophila) [ Homo sapiens ]
• Official Symbol: ASH1L
• Synonyms: ASH1L; ash1 (absent, small, or homeotic)-like (Drosophila); histone-lysine N-methyltransferase ASH1L; ASH1; ASH1L1; huASH1; KMT2H; ASH1-like protein; lysine N-methyltransferase 2H; absent small and homeotic disks protein 1 homolog; probable histone-lysine N-methyltransferase ASH1L; FLJ10504; KIAA1420
• Gene ID: 55870
• mRNA Refseq: NM_018489
• Protein Refseq: NP_060959
• MIM: 607999
• UniProt ID: Q9NR48
• Chromosome Location: 1q22
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; Tight junction, organism-specific biosystem; Tight junction, conserved biosystem
• Function: DNA binding; histone-lysine N-methyltransferase activity; metal ion binding; methyltransferase activity; transferase activity; zinc ion binding