Recombinant Human ATG4B Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ATG4B-4355H
• Product Overview: ATG4B MS Standard C13 and N15-labeled recombinant protein (NP_847896) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 42.4 kDa
• AA Sequence: MDAATLTYDTLRFAEFEDFPETSEP VWILGRKYSIFTEKDEILSDVASRL WFTYRKNFPAIGGTGPTSDTGWGCM LRCGQMIFAQALVCRHLGRDWRWTQ RKRQPDSYFSVLNAFIDRKDSYYSI HQIAQMGVGEGKSIGQWYGPNTVAQ VLKKLAVFDTWSSLAVHIAMDNTVV MEEIRRLCRTSVPCAGATAFPADSD RHCNGFPAGAEVTNRPSPWRPLVLL IPLRLGLTDINEAYVETLKHCFMMP QSLGVIGGKPNSAHYFIGYVGEELI YLDPHTTQPAVEPTDGCFIPDESFH CQHPPCRMSIAELDPSIAVGFFCKT EDDFNDWCQQVKKLSLLGGALPMFE LVELQPSHLACPDVLNLSLGESCQV QILLMTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ATG4B autophagy related 4B cysteine peptidase [ Homo sapiens (human) ]
• Official Symbol: ATG4B
• Synonyms: ATG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); APG4 autophagy 4 homolog B (S. cerevisiae), APG4B; cysteine protease ATG4B; Apg4B; AUTL1; DKFZp586D1822; KIAA0943; hAPG4B; autophagin-1; APG4 autophagy 4 homolog B; AUT-like 1 cysteine endopeptidase; autophagy-related protein 4 homolog B; autophagy-related cysteine endopeptidase 1; APG4B; MGC1353
• Gene ID: 23192
• mRNA Refseq: NM_178326
• Protein Refseq: NP_847896
• MIM: 611338
• UniProt ID: Q9Y4P1