Recombinant Human ATP1B3, His-tagged

• Cat.No.: ATP1B3-184H
• Product Overview: ATP1B3, 57- 279aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 57- 279aa
• Description: ATP1B3 belongs to the X(+)/potassium ATPases subunit beta family. This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-3 subunit is not known. Recombinant human ATP1B3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.15M NaCl, 1mM DTT
• Molecular Mass: 27.4 kDa (246aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSTMWVMLQ TLNDEVPKYR DQIPSPGLMV FPKPVTALEY TFSRSDPTSY AGYIEDLKKF LKPYTLEEQK NLTVCPDGAL FEQKGPVYVA CQFPISLLQA CSGMNDPDFG YSQGNPCILV KMNRIIGLKP EGVPRIDCVS KNEDIPNVAV YPHNGMIDLK YFPYYGKKLH VGYLQPLVAV QVSFAPNNTG KEVTVECKID GSANLKSQDD RDKFLGRVMF KITARA
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ATP1B3 ATPase, Na+/K+ transporting, beta 3 polypeptide [ Homo sapiens ]
• Official Symbol: ATP1B3
• Synonyms: ATP1B3; ATPase, Na+/K+ transporting, beta 3 polypeptide; sodium/potassium-transporting ATPase subunit beta-3; CD298; FLJ29027; Na, K-ATPase beta-3 polypeptide; sodium/potassium-dependent ATPase beta-3 subunit; sodium/potassium-dependent ATPase subunit beta-3; sodium/potassium-transporting ATPase beta-3 chain; ATPB-3
• Gene ID: 483
• mRNA Refseq: NM_001679
• Protein Refseq: NP_001670
• MIM: 601867
• UniProt ID: P54709
• Chromosome Location: 3q23
• Pathway: Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Basigin interactions, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Carbohydrate digestion and absorption, organism-specific biosystem
• Function: sodium:potassium-exchanging ATPase activity