Recombinant Human ATP4A protein, His-tagged

• Cat.No.: ATP4A-19H
• Product Overview: Recombinant Human ATP4A(Leu798~Gly896) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 798-896 a.a.
• Description: The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase.
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 12.7kDa
• AA Sequence: MGHHHHHHSG SEF-LTPYLIY ITVSVPLPLG CITILFIELC TDIFPSVSLA YEKAESDIMH LRPRNPKRDR LVNEPLAAYS YFQIGAIQSF AGFTDYFTAM AQEGWFPLLC VG
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: ATP4A ATPase, H+/K+ exchanging, alpha polypeptide [ Homo sapiens ]
• Official Symbol: ATP4A
• Synonyms: ATP6A
• Gene ID: 495
• mRNA Refseq: NM_000704.2
• Protein Refseq: NP_000695
• MIM: 137216
• UniProt ID: P20648
• Chromosome Location: 19q13.1
• Pathway: Collecting duct acid secretion, organism-specific biosystem;Collecting duct acid secretion, conserved biosystem;Gastric acid secretion, organism-specific biosystem;Gastric acid secretion, conserved biosystem;Ion channel transport, organism-specific biosystem;Ion transport by P-type ATPases, organism-specific biosystem;Oxidative phosphorylation, organism-specific biosystem
• Function: ATP binding;hydrogen:potassium-exchanging ATPase activity;magnesium ion binding;protein heterodimerization activity