| Description : |
Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. This gene encodes a preproprotein that is proteolytically processed to generate a mature azurophil granule antibiotic protein, with monocyte chemotactic and antimicrobial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Form : |
Freeze-dried powder |
| Molecular Mass : |
30kDa as determined by SDS-PAGE reducing conditions. |
| Protein length : |
Pro23~Gly247 (Accession # P20160) |
| Endotoxin : |
<1.0EU per 1ug (determined by the LAL method) |
| Purity : |
>95% |
| Characteristic : |
The isoelectric point is 9.1. |
| Applications : |
SDS-PAGE; WB; ELISA; IP |
| Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
| Storage buffer : |
Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl. |
| Reconstitution : |
Reconstitute in sterile PBS, pH7.2-pH7.4. |
