Recombinant Human B3GALT5 protein, His & GST-tagged

• Cat.No.: B3GALT5-1822H
• Product Overview: Recombinant Human B3GALT5 aa. (Asn29~Val310) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of a family of membrane-bound glycoproteins. The encoded protein may synthesize type 1 Lewis antigens, which are elevated in gastrointestinal and pancreatic cancers. Alternatively spliced transcript variants using multiple alternate promoters have been observed for this gene.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: 63kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Asn29~Val310
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >85%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 9

Gene Information

• Gene Name: B3GALT5 beta-1,3-galactosyltransferase 5 [ Homo sapiens (human) ]
• Official Symbol: B3GALT5
• Synonyms: B3GALT5; beta-1,3-galactosyltransferase 5; B3T5; GLCT5; B3GalTx; B3GalT-V; beta3Gal-T5; beta-3-Gx-T5; beta-1,3-GalTase 5; beta-1,3-galactosyltransferase 5; UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5; homolog of C. elegans Bt toxin resistance gene bre-5
• Gene ID: 10317
• mRNA Refseq: NM_001278650.1
• Protein Refseq: NP_001265579.1
• UniProt ID: Q9Y2C3