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Active Recombinant Human B3GAT1 Protein, His-tagged

Cat.No. : B3GAT1-19H
Product Overview : Recombinant Human B3GAT1(His25-Ile334) fused with His tag at the N-terminus was produced in CHO.
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  • Gene Information
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Description : B3GAT1 is a key enzyme involved in human natural killer1 (HNK1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta 1-3Gal beta 1-4GlcNAc. The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol. The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system. Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method.
Source : CHO
Species : Human
Tag : His
Predicted N Terminal : His
Form : Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Bio-activity : Measured by its ability to transfer GlcA from UDP-GlcA to lactose. The specific activity is >750 pmol/min/μg, as measured under the described conditions.
Molecular Mass : Predicted Molecular Mass: 36 kDa;
SDS-PAGE: 50-59 kDa, reducing conditions.
Protein length : His25-Ile334
Endotoxin : <0.01 EU per 1 μg of the protein by the LAL method.
Purity : >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Shipping : The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Gene Name : B3GAT1 beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) [ Homo sapiens ]
Official Symbol : B3GAT1
Synonyms : B3GAT1; beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); CD57, CD57 antigen , LEU7; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT P; HNK 1; NK 1; glcUAT-P; LEU7 antigen; glucuronosyltransferase P; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP;
Gene ID : 27087
mRNA Refseq : NM_018644
Protein Refseq : NP_061114
MIM : 151290
UniProt ID : Q9P2W7

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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