|Product Overview:||Recombinant Human B3GAT1(His25-Ile334) fused with His tag at the N-terminus was produced in CHO.|
|Description:||B3GAT1 is a key enzyme involved in human natural killer1 (HNK1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta 1-3Gal beta 1-4GlcNAc. The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol. The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system. Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method.|
|Predicted N Terminal:||His|
|Form:||Supplied as a 0.2 μm filtered solution in Tris and NaCl.|
|Bio-activity:||Measured by its ability to transfer GlcA from UDP-GlcA to lactose. The specific activity is >750 pmol/min/μg, as measured under the described conditions.|
|Molecular Mass:||Predicted Molecular Mass: 36 kDa;
SDS-PAGE: 50-59 kDa, reducing conditions.
|Endotoxin:||<0.01 EU per 1 μg of the protein by the LAL method.|
|Purity:||>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
|Shipping:||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Gene Name:||B3GAT1 beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) [ Homo sapiens ]|
|Synonyms:||B3GAT1; beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); CD57, CD57 antigen , LEU7; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT P; HNK 1; NK 1; glcUAT-P; LEU7 antigen; glucuronosyltransferase P; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP;|