Recombinant Human B3GNT3 Protein, His-tagged

Cat.No. : B3GNT3-291H
Product Overview : Recombinant Human B3GNT3 Protein (Leu165-Arg277) with N-His tag was expressed in E. coli.
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Description : This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. It plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 16.8 kDa
Accurate Molecular Mass: 15 kDa
Protein length : Leu165-Arg277
Purity : > 90%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Storage Buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name : B3GNT3 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 [ Homo sapiens (human) ]
Official Symbol : B3GNT3
Synonyms : B3GNT3; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3; TMEM3; B3GN T3; B3GNT 3; beta 1; 3 N acetylglucosaminyltransferase bGnT 3; beta3Gn T3; HP10328; putative type II membrane protein; transmembrane protein 3; beta3GalT8; beta-3-Gx-T8; beta-1,3-Gn-T3; beta-1,3-GalTase 8; core1-beta3GlcNAcT; beta-1,3-N-acetylglucosaminyltransferase bGnT-3; UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8; core 1 extending beta-1,3-N-acetylglucosaminyltransferase; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 8; B3GN-T3; B3GNT-3; B3GAL-T8; beta3Gn-T3
Gene ID : 10331
mRNA Refseq : NM_014256
Protein Refseq : NP_055071
MIM : 605863
UniProt ID : Q9Y2A9

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