| Description : |
Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. In these 2 complexes, it is probably required to maintain the stability of BABAM2 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36 component. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression. Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
36.6 kDa |
| AA Sequence : |
MEVAEPSSPTEEEEEEEEHSAEPRP RTRSNPEGAEDRAVGAQASVGSRSE GEGEAASADDGSLNTSGAGPKSWQV PPPAPEVQIRTPRVNCPEKVIICLD LSEEMSLPKLESFNGSKTNALNVSQ KMIEMFVRTKHKIDKSHEFALVVVN DDTAWLSGLTSDPRELCSCLYDLET ASCSTFNLEGLFSLIQQKTELPVTE NVQTIPPPYVVRTILVYSRPPCQPQ FSLTEPMKKMFQCPYFFFDVVYIHN GTEEKEEEMSWKDMFAFMGSLDTKG TSYKYEVALAGPALELHNCMAKLLA HPLQRPCQSHASYSLLEEEDEAIEV EATVTRTRPLEQKLISEEDLAANDI LDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
