Active Recombinant Human BACE1 protein, Fc-tagged

• Cat.No.: BACE1-002H
• Product Overview: Recombinant Human BACE1 protein(NP_036236.1)(Thr22-Thr457) is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 22-457 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is > 3 pmol/min/µg.
• Molecular Mass: The protein has a predicted MW of 75.2 kDa (pro-form) and 72.6 kDa (mature-form). Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: BACE1 beta-site APP-cleaving enzyme 1 [ Homo sapiens ]
• Official Symbol: BACE1
• Synonyms: BACE1; beta-site APP-cleaving enzyme 1; BACE, beta site APP cleaving enzyme; beta-secretase 1; asp 2; memapsin-2; APP beta-secretase; aspartyl protease 2; beta-site APP cleaving enzyme 1; beta-secretase 1 precursor variant 1; transmembrane aspartic proteinase Asp2; membrane-associated aspartic protease 2; beta-site amyloid beta A4 precursor protein-cleaving enzyme; ASP2; BACE; HSPC104; FLJ90568; KIAA1149
• Gene ID: 23621
• mRNA Refseq: NM_001207048
• Protein Refseq: NP_001193977
• MIM: 604252
• UniProt ID: P56817