Recombinant Human BAG3 Protein, His-tagged

• Cat.No.: BAG3-293H
• Product Overview: Recombinant Human BAG3 Protein (Gly421-Ala498) with N-His tag was expressed in E. coli.

Specification

• Description: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 12.6 kDa
  Accurate Molecular Mass: 15 kDa
• Protein length: Gly421-Ala498
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: BAG3 BCL2-associated athanogene 3 [ Homo sapiens (human) ]
• Official Symbol: BAG3
• Synonyms: BAG3; BCL2-associated athanogene 3; BAG family molecular chaperone regulator 3; docking protein CAIR-1; BCL2-binding athanogene 3; bcl-2-binding protein Bis; bcl-2-associated athanogene 3; BAG-family molecular chaperone regulator-3; BIS; BAG-3; CAIR-1; MGC104307
• Gene ID: 9531
• mRNA Refseq: NM_004281
• Protein Refseq: NP_004272
• MIM: 603883
• UniProt ID: O95817

Reviews

09/14/2021

With its wide applicability, it can play a key role in many fields of research and promote the progress of science.

03/20/2021

The short half-life and high clearance properties of this recombinant protein make it a higher potential in drug research and development, which is expected to become a new generation of therapeutic drugs.

11/06/2020

It's the most biologically active protein I've ever seen, and the purity of its production is impressive.

Q&As

How to quantitatively detect the expression level of BAG3 protein?

The expression level of BAG3 protein can be quantitatively detected by Western blot, immunohistochemistry, real-time PCR and other technical methods.

What is the role of BAG3 protein in cardiomyopathy?

This protein is involved in biological processes such as cardiomyocyte survival and metabolism, and plays an important role in the occurrence and progression of cardiomyopathy.

How can BAG3 protein be used to develop treatments for cardiomyopathy?

The specific regulatory mechanism of BAG3 protein can be explored to develop therapeutic strategies for its function, such as bioengineering drugs or cell gene therapy.

How about the BAG3 protein in amyotrophic lateral sclerosis?

BAG3 protein plays an important role in regulating muscle cell life activities, metabolic processes and protein synthesis, and plays an important role in the occurrence and development of amyotrophic lateral sclerosis.

What is the relationship between BAG3 protein and the autophagy process?

BAG3 protein plays an important role in the autophagy process, such as participating in the formation of autophagy membranes and inhibiting specific steps of autophagy pathways, affecting the occurrence and development of autophagy processes.

How to develop tumor therapeutics for BAG3 protein?

It is possible to develop specific targeted drugs for BAG3 protein, interfere with the binding of BAG3 protein to other apoptosis-regulatory proteins, etc., to affect the growth and metastasis of tumor cells.