| Description : |
This gene encodes a member of the IMD (IRSp53/MIM homology domain) family. Members of this family can be subdivided in two groups, the IRSp53-like and MIM-like, based on the presence or absence of the SH3 (Src homology 3) domain. The protein encoded by this gene contains a conserved IMD, also known as F-actin bundling domain, at the N-terminus, and a canonical SH3 domain near the C-terminus, so it belongs to the IRSp53-like group. This protein is the substrate for insulin receptor tyrosine kinase and binds to the small GTPase Rac. It is involved in signal transduction pathways that link deformation of the plasma membrane and remodeling of the actin cytoskeleton. It also promotes actin assembly and membrane protrusions when overexpressed in mammalian cells, and is essential to the formation of a potent actin assembly complex during EHEC (Enterohemorrhagic Escherichia coli) pedestal formation. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
56.9 kDa |
| AA Sequence : |
MSRGPEEVNRLTESTYRNVMEQFNP GLRNLINLGKNYEKAVNAMILAGKA YYDGVAKIGEIATGSPVSTELGHVL IEISSTHKKLNESLDENFKKFHKEI IHELEKKIELDVKYMNATLKRYQTE HKNKLESLEKSQAELKKIRRKSQGS RNALKYEHKEIEYVETVTSRQSEIQ KFIADGCKEALLEEKRRFCFLVDKH CGFANHIHYYHLQSAELLNSKLPRW QETCVDAIKVPEKIMNMIEEIKTPA STPVSGTPQASPMIERSNVVRKDYD TLSKCSPKMPPAPSGRAYTSPLIDM FNNPATAAPNSQRVNNSTGTSEDPS LQRSVSVATGLNMMKKQKVKTIFPH TAGSNKTLLSFAQGDVITLLIPEEK DGWLYGEHDVSKARGWFPSSYTKLL EENETEAVTVPTPSPTPVRSISTVN LSENSSVVIPPPDYLECLSMGAAAD RRADSARTTSTFKAPASKPETAAPN DANGTAKPPFLSGENPFATVKLRPT VTNDRSAPIIRTRTRPLEQKLISEE DLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
