Recombinant Human BCL11A protein, His-tagged

• Cat.No.: BCL11A-1241H
• Product Overview: Recombinant Human BCL11A aa. (Met1~Arg210 fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Met1~Arg210
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: BCL11A B-cell CLL/lymphoma 11A [ Homo sapiens (human) ]
• Official Symbol: BCL11A
• Synonyms: BCL11A; B-cell CLL/lymphoma 11A; EVI9; CTIP1; DILOS; ZNF856; HBFQTL5; BCL11A-L; BCL11A-S; BCL11a-M; BCL11A-XL; B-cell lymphoma/leukemia 11A; B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2; BCL-11A; BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1; C2H2-type zinc finger protein; COUP-TF-interacting protein 1; EVI-9; ecotropic viral integration site 9 homolog; ecotropic viral integration site 9 protein homolog; zinc finger protein 856
• Gene ID: 53335
• mRNA Refseq: NM_018014.3
• Protein Refseq: NP_060484.2
• UniProt ID: Q9H165