Recombinant Human BDNF protein, For Organoid Culture

• Cat.No.: BDNF-4565H
• Product Overview: Recombinant Human BDNF protein(P23560)(His129-Arg247) is expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: His129-Arg247
• Tag: Non
• Form: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
• Bio-activity: Immobilized Human TrkB (C-6His) at 5 μg/mL (100 μL/well) can bind Human/Murine/Rat BDNF: Biotinylated by NHS-biotin prior to testing. The EC50 of Human/Murine/Rat BDNF is ≤20 ng/mL.
• Molecular Mass: 13 KDa
• Endotoxin: < 0.01 EU/μg as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.