Recombinant Human beclin 1, autophagy related, His-tagged

• Cat.No.: BECN1-377H
• Product Overview: BECN1, 1-450aa, Human, His tag, E.coli

Specification

• Description: BECN1, also as known as Beclin-1 and autophagy-related gene (Atg) 6, belongs to the beclin family. This protein plays a central role in autophagy. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagasome in autophagy. Also it required for the abcission step of cytokinesis. BECN1 plays a role in antiviral host defense. Recombinant human BECN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.5) containing 0.1M NaCl, 10% glycerol,1mM DTT
• Molecular Mass: 54.1 kDa (470aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: BECN1 beclin 1, autophagy related [ Homo sapiens ]
• Official Symbol: BECN1
• Synonyms: BECN1; beclin 1, autophagy related; beclin 1 (coiled coil, moesin like BCL2 interacting protein); beclin-1; ATG6; ATG6 autophagy related 6 homolog (S. cerevisiae); VPS30; ATG6 autophagy related 6 homolog; coiled-coil myosin-like BCL2-interacting protein; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); beclin1
• Gene ID: 8678
• mRNA Refseq: NM_003766
• Protein Refseq: NP_003757
• MIM: 604378
• UniProt ID: Q14457
• Chromosome Location: 17q21
• Pathway: Regulation of autophagy, organism-specific biosystem; Regulation of autophagy, conserved biosystem; Senescence and Autophagy, organism-specific biosystem
• Function: protein binding